Interleukin-I (22-K Factor) Induces Release of Granulocyte-Macrophage Colony-Stimulating Activity From Human Mononuclear Phagocytes
نویسندگان
چکیده
An electrophoretically pure preparation of natural human interleukin-1 (IL-i ) was shown to stimulate in vitro colony formation in human bone marrow cultures. Day 4 myeloid cluster-forming cells (CFC). as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (CFU-GM) were stimulated in a dose-dependent fashion. At optimal concentrations of IL-i . the number of day 4 CFC reached 72%. the number of day 7 CFU-GM reached 32%. and the number of day 1 0 CFU-GM reached 80% of the respective numbers of colonies obtained by addition of crude leukocyte-conditioned medium (1CM). The IL-i induced stimulatory effect on CFU-GM growth could be completely neutralized by a rabbit anti-IL-i antiserum. Colony growth was abrogated by depleting the marrow cell
منابع مشابه
Interleukin-1 (22-K factor) induces release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes.
An electrophoretically pure preparation of natural human interleukin-1 (IL-1) was shown to stimulate in vitro colony formation in human bone marrow cultures. Day 4 myeloid cluster-forming cells (CFC), as well as early (day 7) and late (day 10) granulocyte-macrophage colony-forming units (CFU-GM) were stimulated in a dose-dependent fashion. At optimal concentrations of IL-1, the number of day 4 ...
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